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KMID : 1134820090380111618
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Journal of the Korean Society of Food Science and Nutrition
2009 Volume.38 No. 11 p.1618 ~ p.1624
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Development of a Predictive Growth Model of Staphylococcus aureus and Shelf-life Estimation of Cooked Mung Bean Sprouts Served in School Foodservice Operations
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Park Hyung-Soo
Kim Min-Young
Jeong Hyun-Suk
Park Ki-Hwan
Ryu Kyung
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Abstract
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This study was conducted to estimate the shelf-life of cooked mung bean sprouts contaminated with Staphylococcus aureus according to storage temperatures after cooking in school foodservice operations. A predictive growth model of S. aureus in cooked mung bean sprouts prepared using a standard recipe was developed at 4 storage temperatures (5, 15, 25, and 35℃). To determine the effect of vinegar on the shelf-life of cooked mung bean sprouts, the growth of S. aureus in sprouts prepared using vinegar and the standard recipe were compared. The R² values of the specific growth rate (SGR) and lag time (LT) determined using the Gompertz model were greater than 0.90 at all temperatures except 5℃, which confirmed that it would be appropriate to use these parameters for a secondary model. The secondary model, which indicates changes in LT and SGR values according to storage temperatures, was calculated using response surface models. The compatibility of the developed model was confirmed by calculating R², Bf, Af and MSE values as statistic parameters. The R² values of LT and SGR were 0.94 or higher, and the MSE, Bf and Af values were 0.02 and 0.002, 0.97 and 1.03, and 1.31 and 1.10, respectively, with high statistical compatibility. The growth rate of S. aureus was higher when the standard recipe was used than when vinegar was used at all temperatures. Indeed, no growth of S. aureus was observed in mung bean sprouts prepared using vinegar. Based on the model developed, cooked mung bean sprouts prepared using the standard recipe for school foodservice should be stored at 10℃or less. Additionally, sprouts stored at 25 or 35℃ should be consumed within 6 or 12 hours after cooking. Finally, the addition of vinegar will prevent the growth of S. aureus in cooked mung bean sprouts.
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KEYWORD
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cooked mung bean sprout, Staphylococcus aureus, predictive model, shelf-life, school foodservice
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